Seybold Report ISSN: 1533-9211
1.Archana R. Dhole*, Sandeep R. Kane, Trupti P. Lade, Manisha M Murgude, 2.Virendra C. Yeligar, 3.Vikas R. Dhole, 4Pravinkumar. D. Lade
Vol 17, No 12 ( 2022 ) | Licensing: CC 4.0 | Pg no: 3191-3226 | Published on: 22-12-2022
Abstract
This study is aimed to investigate the protective effect of Herbal and polyherbal formulation against ethylene glycol (EG) induced urolithiasis in Wistar rats. The protective effect was evaluated using EG-induced urolithiasis in rats. All the extracts were studied for in-vitro activity with the nucleation assay, Growth Assay, Cell Culture, Cytotoxicity - Trypan Blue Assay, LDH Leakage Assay for effective inhibition of crystal growth. The combination of extract shows better results for inhibition of growth. The results indicate that administration of extracts and inhibit the growth of urinary stones. It is also seen that the prophylactic effect is more efficient than the curative effect. Therefore, the extracts is useful to prevent the recurrence of urolithiasis as it proved its effect on the early stages of stone development. Related to increased diuresis and lowering of urinary concentrations of stone-forming components.
Keywords:
Ethylene glycol, Wistar rats; Polyherbal formulation, Cytotoxicity -Trypan Blue Assay, LDH Leakage Assay, Urolithiasis