Seybold Report ISSN: 1533-9211
Westeous Dominic Pereira
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai -77, Email: 151301101.sdc@saveetha.com
Anitha Roy
Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai -77, Email: 151301101.sdc@saveetha.com
S.Rajeshkumar
Associate Professor, Department of Pharmacology, Saveetha Dental College and Hospitals
Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University
Chennai -77, Email: rajeshkumars.sdc@saveetha.com
Vol 17, No 09 ( 2022 ) | DOI: 10.5281/zenodo.7089072 | Licensing: CC 4.0 | Pg no:1058-1072 | Published on: 17-09-2022
Abstract
Aim and Background: Nanotechnology is one of the most rapidly developing topics in biotechnology, having a wide range of potential uses. Nanoparticles have transformed a variety of fields including health, dietary habits, and energy. Nanotechnology’s application in medicine, especially drug delivery, has demonstrated several advantages. Nanoparticles are being used to reduce the side-effects and to eliminate the consequences that drugs may have on the patient. The aim of this study is to create copper oxide nanoparticles out of Cassia oleoresin and test their antimicrobial and cytotoxicity properties once they have been characterized.
Materials and Method: The DPPH free radical scavenging activity of cassia oleoresin was evaluated using CuNPs in a DPPH test. Antimicrobial activity was observed using the Agar well diffusion technique, while cytotoxic activity was measured using ELISA plates containing brine shrimp nauplii.
Result: Cassia oleoresins were easily biosynthesized and had antioxidant, antibacterial, and cytotoxic property that was equivalent to standard. The production of nanoparticles had a peak at 350 nanometers, according to a graphical representation. Candida albicans was used to assess the antifungal activity, and at a concentration of 150µl, it showed the greatest inhibition zone. Antimicrobial activity was tested against oral pathogens Streptococcus mutans and Lactobacillus, with a maximal zone of inhibition seen at 150 µl. Antifungal and antibacterial action is represented by inhibition zones. In concentrations 5µL, 10µL, 15µL and 25µL, 100 percent of living brine shrimps were found, whereas in concentrations of 25µL, the proportion of alive brine shrimp was 80 percent.
Conclusion: As a result, it can be concluded that nanoparticles mediated by cassia oleoresin oil have the potential to be implemented as an efficient antioxidant, antibacterial, and cytotoxic agent. hence it can be used in large-scale manufacturing and in a variety of medical applications.
Keywords:
Cassia Oleoresin, CuNPs, Biosynthesis of nanoparticles, antioxidant activity.