Seybold Report ISSN: 1533-9211
Keerthika .R.
Department of Conservative dentistry and Endodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical And Technical Sciences, Saveetha University, Chennai, India, Mail id : keerthikar91@gmail.com
Dr.Sandhya Raghu
Professor, Department of Conservative Dentistry and Endodontics, Saveetha Dental College and hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University
Chennai-77, India, Email id: sandhya.sdc@saveetha.com
Vol 17, No 09 ( 2022 ) | DOI: 10.5281/zenodo.7095006 | Licensing: CC 4.0 | Pg no:1105-1114 | Published on: 20-09-2022
Abstract
Increasing emergence of resistance to the currently available antibiotics has necessitated continued search for new antimicrobial compounds.Gymnema sylvestre is a woody vine common in tropical areas of Asia, Africa and Australia and it is believed that this plant possesses antiviral, antibacterial, anti-allergic, anti-oxidants and hypoglycemic properties.Thus this study aims to evaluate the antibacterial efficacy of Gymnema sylvestre against Enterococcus faecalis biofilm.
Materials and method
Ethanolic extracts prepared from Gymnema sylvestre leaves were screened for in vitro antimicrobial activity against Enterococcus faecalis using disk diffusion method. For statistical analysis of data, multiple comparisons were performed using one-way analysis of variance (ANOVA) followed by the LSD test for post hoc analysis. Statistical significance was accepted at a level of P 0.05. Data were analyzed using SPSS (version 11).
Results
A dose dependent antibacterial effect of extracts of Gymnema sylvestre leaf was noted with maximum antibacterial effect at the concentration of 50 µg/ml.
Conclusion
Ethanolic extract of Gymnema sylvestre demonstrated antimicrobial activity against Enterococcus faecalis recommending further invivo and toxicology studies to prove its use in the clinical situation.
Keywords:
Gymnema sylvestre ,Antimicrobial activity ,Enterococcus faecalis ,Disk diffusion method.